Pto is a serine/threonine kinase that confers resistance in tomato to strains of Pseudomonas spp. that express the avirulence proteins AvrPto or AvrPtoB. Pto interacts with AvrPto and AvrPtoB in the yeast two-hybrid system and elicits a form of cell death, the hypersensitive response (HR) when coexpressed with them in planta. We are using DNA shuffling to identify functional regions of Pto for interaction with the avr proteins in yeast and signaling downstream in planta. A library of chimeras was generated by DNA shuffling of Pto and four of its paralogs from a resistant haplotype of tomato. A subset of these chimeras was further phenotyped for interaction with the avr proteins in yeast and signaling downstream for an HR in planta.
This mySQL database was created as a set of tools to manage and visualize data from DNA shuffling experiments of the Pto disease resistance gene and homologous sequences in tomato. Several of the tools generated here will be useful for analysis of data from DNA shuffling of other gene families.
To use these tools on our data, click on the Data Analysis icon. Scripts for querying the database are available on request.
To download specific programs click here.
This project is funded by the NSF Cooperative Agreement BIR-8920216 to CEPRAP and the NSF Integrative Plant Biology Program award # 0133993
Supplemental Data to the paper:
Functional analysis of the plant disease resistance gene Pto using DNA shuffling
Adriana J. Bernal, Qilin Pan, Jeff Pollack, Laura Rose, Alexander Kozik, Neil Willits, Yao Luo, Muriel Guittet, Elena Kochetkova, Richard W. Michelmore.
protein alignment of shuffled clones
alignments with selected clones (constitutively active molecules in planta, clones that only interact with AvrPto and clones that only interact with AvrPtoB)
results of recombination analysis
phenotype data for analyzed recombinants
joint table (phenotype + alignment) for analyzed recombinants